biochemical characterization of a novel thermophilic esterase isolated from shewanella sp f88

Biochemical Characterization of A Novel Thermophilic Esterase Isolated from Shewanella sp F88

The main objective of this study was to purify and characterize an esterase from Shewanella sp F88. The enzyme was purified 41-fold and an overall yield of 21 %, using a two-step procedure, including ammonium sulfate precipitation and Q-sepharore chromatography. Molecular weight of the enzyme was 62.3 kDa according to SDS-PAGE data. The enzyme showed an optimum activity at pH 6.5 and 58 ˚C. Evo...

Thermotoga lettingae sp. nov., a novel thermophilic, methanol-degrading bacterium isolated from a thermophilic anaerobic reactor.

A novel, anaerobic, non-spore-forming, mobile, Gram-negative, thermophilic bacterium, strain TMOT, was isolated from a thermophilic sulfate-reducing bioreactor operated at 65 C with methanol as the sole substrate. The G+C content of the DNA of strain TMOT was 39.2 mol%. The optimum pH, NaCl concentration, and temperature for growth were 7.0, 1.0%, and 65 degrees C, respectively. Strain TMOT was...

Purification and characterization of an acidic, thermophilic phytase from a newly isolated Geobacillus stearothermophilus strain DM12

Microbial phytases were applied mainly to animal and human foodstuffs in order to improvemineral bioavailability and food processing. In addition, phytases have potentialbiotechnological application in various other fields, such as environmental protection,aquaculture and agriculture. Bacillus sp. DM12, an isolate from a hot spring, produces phytase,which catalyzes the hydrolysis of phytic acid...

Shewanella psychrophila sp. nov. and Shewanella piezotolerans sp. nov., isolated from west Pacific deep-sea sediment.

Two Shewanella-like bacterial strains, WP2(T) and WP3(T), which were isolated from west Pacific deep-sea sediment, were studied to determine their taxonomic position. Cells of the two bacteria were facultatively anaerobic, Gram-negative rods and motile by means of a single polar flagellum. Strain WP2(T) was psychrophilic, growing optimally at about 10-15 degrees C, whereas strain WP3(T) was psy...

Biochemical characterization of an enantioselective esterase from Brevundimonas sp. LY-2

BACKGROUND Lactofen, a member of the diphenylether herbicides, has high activity and is commonly used to control broadleaf weeds. As a post-emergent herbicide, it is directly released to the environment, and easily caused the pollution. This herbicide is degraded in soil mainly by microbial activity, but the functional enzyme involved in the biodegradation of lactofen is still not clear now. ...

Biochemical characterization of thermophilic actinomycete (Streptomyces sp. MSC702) from mushroom compost in India